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Biophys J. 2015 Mar 24;108(6):1330-1340. doi: 10.1016/j.bpj.2015.01.016.

Structural, mechanical, and dynamical variability of the actin cortex in living cells.

Author information

1
U1006 INSERM, Université Aix-Marseille, Parc Scientifique et Technologique de Luminy, Marseille, France.
2
U1006 INSERM, Université Aix-Marseille, Parc Scientifique et Technologique de Luminy, Marseille, France. Electronic address: simon.scheuring@inserm.fr.

Abstract

In eukaryotic cells, an actin-based cortex lines the inner leaflet of the plasma membrane, endowing the cells with crucial mechanical and functional properties. Unfortunately, it has not been possible to study the structural dynamics of the actin cortex at high lateral resolution in living cells. Here, we performed atomic force microscopy time-lapse imaging and mechanical mapping of actin in the cortex of living cells at high lateral and temporal resolution. Cortical actin filaments adopted discernible arrangements, ranging from large parallel bundles with low connectivity to a tight meshwork of short filaments. Mixing of these architectures resulted in attuned cortex networks with specific connectivity, mechanical responses, and marked differences in their dynamic behavior.

PMID:
25809247
PMCID:
PMC4375525
DOI:
10.1016/j.bpj.2015.01.016
[Indexed for MEDLINE]
Free PMC Article

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