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Environ Microbiol. 2015 Nov;17(11):4332-51. doi: 10.1111/1462-2920.12856. Epub 2015 Apr 28.

Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections.

Author information

1
Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora', Facultad de Ciencias, Universidad de Málaga-CSIC (IHSM-UMA-CSIC), Área de Genética, Campus de Teatinos, Málaga, E-29010, Spain.
2
Dpto. Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, Granada, Spain.
3
Department of Life Sciences, MRC Centre for Molecular Bacteriology and Infection, Imperial College London, South Kensington Campus, Flowers Building, London, SW7 2AZ, UK.
4
Pseudomonas aeruginosa host-pathogen translational research group, Lille School of Medicine, UDSL, Lille North of France University, Lille, France.

Abstract

The second messenger cyclic di-GMP (c-di-GMP) controls the transition between different lifestyles in bacterial pathogens. Here, we report the identification of DgcP (diguanylate cyclase conserved in Pseudomonads), whose activity in the olive tree pathogen Pseudomonas savastanoi pv. savastanoi is dependent on the integrity of its GGDEF domain. Furthermore, deletion of the dgcP gene revealed that DgcP negatively regulates motility and positively controls biofilm formation in both the olive tree pathogen P. savastanoi pv. savastanoi and the human opportunistic pathogen Pseudomonas aeruginosa. Overexpression of the dgcP gene in P. aeruginosa PAK led to increased exopolysaccharide production and upregulation of the type VI secretion system; in turn, it repressed the type III secretion system, which is a hallmark of chronic infections and persistence for P. aeruginosa. Deletion of the dgcP gene in P. savastanoi pv. savastanoi NCPPB 3335 and P. aeruginosa PAK reduced their virulence in olive plants and in a mouse acute lung injury model respectively. Our results show that diguanylate cyclase DgcP is a conserved Pseudomonas protein with a role in virulence, and confirm the existence of common c-di-GMP signalling pathways that are capable of regulating plant and human Pseudomonas spp. infections.

PMID:
25809128
DOI:
10.1111/1462-2920.12856
[Indexed for MEDLINE]
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