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Poult Sci. 2015 May;94(5):947-54. doi: 10.3382/ps/pev086. Epub 2015 Mar 23.

Isolation of chicken astrovirus from specific pathogen-free chicken embryonated eggs.

Author information

1
Department of Pathology, School of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando M. Paiva, 87, 05508-270, São Paulo, SP, Brazil.
2
Laboratory of Electron Microscopy, Center for Research and Development of Animal Health, InstitutoBiológico, Av. Cons. Rodrigues Alves, 1252, 04014-002, São Paulo, SP, Brazil.
3
Department of Pathology, School of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando M. Paiva, 87, 05508-270, São Paulo, SP, Brazil ajpferr@usp.br.

Abstract

Astroviruses have been associated with enteric disorders in many animal species, including chickens. Here, we describe the isolation, propagation, and pathological characteristics of chicken astrovirus (CAstV) in specific pathogen free (SPF) chicken embryonated eggs (CEE) from chickens with diarrhea and runting-stunting syndrome. The CEE were inoculated via the yolk sac route. Viral confirmation was carried out using PCR techniques and transmission electron microscopy negative staining with ammonium molybdate. The intestinal contents were screened for CAstV, and differential diagnostic testing was performed for avian nephritis virus, avian rotavirus, avian reovirus, chicken parvovirus, infectious bronchitis virus, and fowl adenovirus Group I to detect co-infection with other infectious agents. Seven- or 14-day-old CEEs presented with hemorrhages, edema, a gelatinous aspect, deformities, and dwarfism. The supporting membranes did not show any alterations. Here, we have described the isolation of CAstV and its pathological characteristics in SPF CEE.

KEYWORDS:

chicken astrovirus; chicken embryonated eggs; enteric virus; viral isolation

PMID:
25805833
DOI:
10.3382/ps/pev086
[Indexed for MEDLINE]

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