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Anal Biochem. 1985 Jan;144(1):253-7.

An assay for GDP-D-mannose-4,6-dehydratase.

Abstract

The mechanism of GDP-D-mannose-4,6-dehydratase action with respect to loss of the C5 hydrogen has been established using GDP-D-[5-3H]-mannose as a substrate. This observation has been incorporated into a rapid assay for the enzyme based on the equilibration of 3H with the aqueous medium.

PMID:
2580455
[Indexed for MEDLINE]
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