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J Biotechnol. 2015 Jun 10;203:19-21. doi: 10.1016/j.jbiotec.2015.03.005. Epub 2015 Mar 20.

Improved genome sequence of the phytopathogenic fungus Rhizoctonia solani AG1-IB 7/3/14 as established by deep mate-pair sequencing on the MiSeq (Illumina) system.

Author information

1
Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld University, D-33501 Bielefeld, Germany.
2
Bioinformatics and Systems Biology, Gießen University, D-35392 Gießen, Germany.
3
Leibniz-Institute of Vegetables and Ornamental Crops, D-14979 Großbeeren, Germany.
4
Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld University, D-33501 Bielefeld, Germany. Electronic address: aschluet@CeBiTec.Uni-Bielefeld.de.

Abstract

The phytopathogenic fungus Rhizoctonia solani AG1-IB of the phylum Basidiomycota affects various economically important crops comprising bean, rice, soybean, figs, cabbage and lettuce. The R. solani isolate 7/3/14 of the anastomosis group AG1-IB was deeply resequenced on the Illumina MiSeq system applying the mate-pair mode to improve its genome sequence. Assembly of obtained sequence reads significantly reduced the amount of scaffolds and improved the genome sequence of the isolate compared to the previous sequencing approach. The genome sequence of the AG1-IB isolate 7/3/14 now provides an up-graded basis to analyze genome features predicted to play a role in pathogenesis and for the development of strategies to antagonize the pathogenic impact of this fungus.

KEYWORDS:

AUGUSTUS; Plant pathogenic fungus; Rhizoctonia solani AG1-IB; Ultrafast sequencing

PMID:
25801332
DOI:
10.1016/j.jbiotec.2015.03.005
[Indexed for MEDLINE]

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