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Nucleic Acids Res. 2015 Apr 20;43(7):3726-35. doi: 10.1093/nar/gkv235. Epub 2015 Mar 23.

A model for transcription initiation in human mitochondria.

Author information

1
Department of Cell Biology, School of Osteopathic Medicine, Rowan University, 2 Medical Center Dr., Stratford, NJ 08084, USA.
2
Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK.
3
Max Planck Institute for Biophysical Chemistry, Department of Molecular Biology, Am Fassberg 11, 37077 Gottingen, Germany.
4
Department of Cell Biology, School of Osteopathic Medicine, Rowan University, 2 Medical Center Dr., Stratford, NJ 08084, USA d.temiakov@rowan.edu.

Abstract

Regulation of transcription of mtDNA is thought to be crucial for maintenance of redox potential and vitality of the cell but is poorly understood at the molecular level. In this study we mapped the binding sites of the core transcription initiation factors TFAM and TFB2M on human mitochondrial RNA polymerase, and interactions of the latter with promoter DNA. This allowed us to construct a detailed structural model, which displays a remarkable level of interaction between the components of the initiation complex (IC). The architecture of the mitochondrial IC suggests mechanisms of promoter binding and recognition that are distinct from the mechanisms found in RNAPs operating in all domains of life, and illuminates strategies of transcription regulation developed at the very early stages of evolution of gene expression.

PMID:
25800739
PMCID:
PMC4402542
DOI:
10.1093/nar/gkv235
[Indexed for MEDLINE]
Free PMC Article

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