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Biomed Opt Express. 2015 Feb 19;6(3):849-58. doi: 10.1364/BOE.6.000849. eCollection 2015 Mar 1.

Reagent- and separation-free measurements of urine creatinine concentration using stamping surface enhanced Raman scattering (S-SERS).

Author information

1
Department of Electrical & Computer Engineering, University of Houston, 4800 Calhoun Rd., Houston, Texas 77024, USA.
2
Department of Biomedical Engineering, University of Houston, 4800 Calhoun Rd., Houston, Texas 77024, USA.
3
Department of Electrical & Computer Engineering, University of Houston, 4800 Calhoun Rd., Houston, Texas 77024, USA ; Department of Biomedical Engineering, University of Houston, 4800 Calhoun Rd., Houston, Texas 77024, USA.

Abstract

We report a novel reagent- and separation-free method for urine creatinine concentration measurement using stamping surface enhanced Raman scattering (S-SERS) technique with nanoporous gold disk (NPGD) plasmonic substrates, a label-free, multiplexed molecular sensing and imaging technique recently developed by us. The performance of this new technology is evaluated by the detection and quantification of creatinine spiked in three different liquids: creatinine in water, mixture of creatinine and urea in water, and creatinine in artificial urine within physiologically relevant concentration ranges. Moreover, the potential application of our method is demonstrated by creatinine concentration measurements in urine samples collected from a mouse model of nephritis. The limit of detection of creatinine was 13.2 nM (0.15 µg/dl) and 0.68 mg/dl in water and urine, respectively. Our method would provide an alternative tool for rapid, cost-effective, and reliable urine analysis for non-invasive diagnosis and monitoring of renal function.

KEYWORDS:

(170.5660) Raman spectroscopy; (240.6695) Surface-enhanced Raman scattering; (280.1415) Biological sensing and sensors

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