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Mol Breed. 2015;35(4):101. Epub 2015 Mar 17.

Development of molecular markers tightly linked to Pvr4 gene in pepper using next-generation sequencing.

Author information

1
Department of Plant Protection, Faculty of Agriculture, University of Akdeniz, Antalya, Turkey.
2
M.Y. Genetic Agriculture Technology Laboratory, Antalya, Turkey.
3
Yüksel Seed, Kurşunlu, Madenler Mahallesi, Antalya, Turkey.
4
Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter, EX4 4QD UK.
5
National Pollen and Aerobiology Research Unit (NPARU), The University of Worcester, Henwick Grove, Worcester, WR2 6 AJ UK.

Abstract

It is imperative to identify highly polymorphic and tightly linked markers of a known trait for molecular marker-assisted selection. Potyvirus resistance 4 (Pvr4) locus in pepper confers resistance to three pathotypes of potato virus Y and to pepper mottle virus. We describe the use of next-generation sequencing technology to generate molecular markers tightly linked to Pvr4. Initially, comparative genomics was carried out, and a syntenic region of tomato on chromosome ten was used to generate PCR-based markers and map Pvr4. Subsequently, the genomic sequence of pepper was used, and more than 5000 single-nucleotide variants (SNVs) were identified within the interval. In addition, we identified nucleotide binding site-leucine-rich repeat-type disease resistance genes within the interval. Several of these SNVs were converted to molecular markers desirable for large-scale molecular breeding programmes.

KEYWORDS:

MAS; Next-generation sequencing; Pepper; Potato virus Y; Pvr4; Synteny

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