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Mol Breed. 2015;35(4):101. Epub 2015 Mar 17.

Development of molecular markers tightly linked to Pvr4 gene in pepper using next-generation sequencing.

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Department of Plant Protection, Faculty of Agriculture, University of Akdeniz, Antalya, Turkey.
M.Y. Genetic Agriculture Technology Laboratory, Antalya, Turkey.
Yüksel Seed, Kurşunlu, Madenler Mahallesi, Antalya, Turkey.
Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter, EX4 4QD UK.
National Pollen and Aerobiology Research Unit (NPARU), The University of Worcester, Henwick Grove, Worcester, WR2 6 AJ UK.


It is imperative to identify highly polymorphic and tightly linked markers of a known trait for molecular marker-assisted selection. Potyvirus resistance 4 (Pvr4) locus in pepper confers resistance to three pathotypes of potato virus Y and to pepper mottle virus. We describe the use of next-generation sequencing technology to generate molecular markers tightly linked to Pvr4. Initially, comparative genomics was carried out, and a syntenic region of tomato on chromosome ten was used to generate PCR-based markers and map Pvr4. Subsequently, the genomic sequence of pepper was used, and more than 5000 single-nucleotide variants (SNVs) were identified within the interval. In addition, we identified nucleotide binding site-leucine-rich repeat-type disease resistance genes within the interval. Several of these SNVs were converted to molecular markers desirable for large-scale molecular breeding programmes.


MAS; Next-generation sequencing; Pepper; Potato virus Y; Pvr4; Synteny

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