Format

Send to

Choose Destination
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Jun 15;145:353-359. doi: 10.1016/j.saa.2015.03.026. Epub 2015 Mar 9.

Multispectral studies of DNA binding, antioxidant and cytotoxic activities of a new pyranochromene derivative.

Author information

1
Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran.
2
Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran. Electronic address: gdehghan@tabrizu.ac.ir.

Abstract

The binding properties of a new pyranochromene derivative, 2-amino-4-(3-hydroxyphenyl)-5-oxo-4H, 5H-pyrano-[3, 2-c] chromene-3-carbonitrile (3-HC) with calf thymus DNA (ctDNA) have been investigated by UV-vis absorption, circular dichroism, fluorescence spectroscopy and viscosity measurement. These results indicated that 3-HC can interact with DNA through non-intercalative mode and the intrinsic binding constant (Kb) for 3-HC with DNA was estimated to be 3.6 × 10(3)M(-1). The antioxidant activity experiments show that 3-HC also exhibit good antioxidant activity in DPPH free radical scavenging and ferric reducing ability methods. Moreover, 3-HC exhibited cytotoxic activity against K562, human chronic myelogenous leukemia cells, with IC50 value of 146 μM and the cells responded to the treatment with mostly through apoptosis.

KEYWORDS:

Antioxidant potential; Cytotoxicity; Outside DNA binding; Pyranochromene

PMID:
25795609
DOI:
10.1016/j.saa.2015.03.026
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center