Format

Send to

Choose Destination
J Biosci Bioeng. 2015 Oct;120(4):438-43. doi: 10.1016/j.jbiosc.2015.02.012. Epub 2015 Mar 17.

Culture characteristics of the atmospheric and room temperature plasma-mutated Spirulina platensis mutants in CO2 aeration culture system for biomass production.

Author information

1
Key Laboratory for Industrial Biocatalysis, Ministry of Education, Beijing 100084, China; Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
2
Department of Biotechnology, Beijing Polytechnic, Beijing 100029, China.
3
Key Laboratory for Industrial Biocatalysis, Ministry of Education, Beijing 100084, China; Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; Biobreeding Research Center, Wuxi Research Institute of Applied Technologies, Tsinghua University, Wuxi 214072, China.
4
Biobreeding Research Center, Wuxi Research Institute of Applied Technologies, Tsinghua University, Wuxi 214072, China; Department of Engineering Physics, Tsinghua University, Beijing 100084, China.
5
Key Laboratory for Industrial Biocatalysis, Ministry of Education, Beijing 100084, China; Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; Biobreeding Research Center, Wuxi Research Institute of Applied Technologies, Tsinghua University, Wuxi 214072, China. Electronic address: xhxing@tsinghua.edu.cn.

Abstract

For biomass production of Spirulina platensis as feedstock of fermentation, the culture characteristics of three typical mutants of 3-A10, 3-B2 and 4-B3 generated by atmospheric and room temperature plasmas (ARTP) mutagenesis were systematically studied by using CO2 aeration culture system and compared with the wild strain. The specific growth rate of wild strain in the pure air aeration culture system exhibited a 76.2% increase compared with static culture, while the specific growth rates of the 3-A10, 3-B2 and 4-B3 in pure air aeration culture system were increased by 114.4%, 95.9% and 88.2% compared with their static cultures. Compared with static culture, the carbohydrate contents of wild strain, 3-A10, 3-B2 and 4-B3 in pure air aeration culture system dropped plainly by 51.0%, 79.3%, 85.5% and 26.1%. Increase of CO2 concentration enhanced carbohydrate content and productivity. Based on the carbohydrate productivity, the optimal inlet of CO2 concentration in aeration culture was determined to be 12% (v/v). Under this condition, 3-B2 exhibited the highest carbohydrate content (30.7%), CO2 fixation rate (0.120gCO2·g(-1)·d(-1)) and higher growth rate (0.093 g L(-1)·d(-1)), while 3-A10 showed the highest growth rate (0.118 g L(-1)·d(-1)) and higher CO2 fixation rate (0.117gCO2·g(-1)·d(-1)) but low carbohydrate content (24.5%), and 4-B3 showed the highest chlorophyll (Chl) content (3.82 mg·g(-1)). The most outstanding mutant by static culture in terms of growth rate and carbohydrate productivity (3-B2), was also demonstrated by CO2 aeration culture system. This study revealed that the ARTP mutagenesis could generate the S. platensis mutants suitable for CO2 aeration culture aiming at biomass production.

KEYWORDS:

Atmospheric and room temperature plasmas mutagenesis; Biomass production; CO(2) aeration culture; CO(2) fixation rate; Carbohydrate content; Spirulina platensis

PMID:
25795571
DOI:
10.1016/j.jbiosc.2015.02.012
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center