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Food Chem. 2015 Aug 15;181:119-26. doi: 10.1016/j.foodchem.2015.02.041. Epub 2015 Feb 14.

Simultaneous determination of 38 veterinary antibiotic residues in raw milk by UPLC-MS/MS.

Author information

1
College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong, China.
2
Ministry of Agriculture - Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Beijing, China; Ministry of Agriculture - Milk and Dairy Product Inspection Center (Beijing), Beijing, China; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
3
Ministry of Agriculture - Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Beijing, China; Ministry of Agriculture - Milk and Dairy Product Inspection Center (Beijing), Beijing, China; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China. Electronic address: wangjiaqinmqc@126.com.

Abstract

A selective and rapid method has been developed to determine, simultaneously, 38 veterinary antibiotic residues in raw milk by ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). One milliliter of raw milk was diluted with 0.5 mL water and 3 mL acetonitrile, then purified using an Oasis HLB cartridge. The eluates were evaporated by nitrogen drying and then reconstituted to 4 mL with water/acetonitrile (8:1) before being injected into the UPLC-MS/MS system. The results indicated recoveries of 68-118% for 14 β-lactams, 79-118% for eight quinolones, 71-106% for eight sulfonamides, 76-116% for four tetracyclines, 78-106% for three macrolides, and 88-103% for one lincosamides, with coefficients of variation less than 15% for intraday and interday precisions. The limit of quantification for all antibiotics was 0.03-10 μg kg(-1). This methodology was then applied to field-collected real raw milk samples and trace levels of four antibiotics were detected.

KEYWORDS:

Multiclass analysis; Raw milk; UPLC–MS/MS; Veterinary antibiotics

PMID:
25794729
DOI:
10.1016/j.foodchem.2015.02.041
[Indexed for MEDLINE]

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