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Gene. 2015 Jun 10;564(1):29-34. doi: 10.1016/j.gene.2015.03.030. Epub 2015 Mar 17.

The association of PLA2G2A single nucleotide polymorphisms with type IIa secretory phospholipase A2 level but not its activity in patients with stable coronary heart disease.

Author information

1
Russian Cardiology Research and Production Complex, Department of Atherosclerosis Problems, 3rd Cherepkovskaya str, 15a, Moscow 121552, Russia. Electronic address: shuvalovaj@mail.ru.
2
Russian Cardiology Research and Production Complex, Laboratory of Medical Genetics, 3rd Cherepkovskaya str, 15a, Moscow 121552, Russia.
3
Russian Cardiology Research and Production Complex, Department of Atherosclerosis Problems, 3rd Cherepkovskaya str, 15a, Moscow 121552, Russia.
4
Russian Cardiology Research and Production Complex, Laboratory of Biochemical Engineering, 3rd Cherepkovskaya str, 15a, Moscow 121552, Russia.
5
Institute of General Genetics named Vavilov N. I., Department of Genetic Safety, Laboratory of Ecological Genetics, Gubkina str, 3, Moscow 117971, Russia.

Abstract

BACKGROUND:

Single nucleotide polymorphisms (SNPs) of the secretory phospholipase A2 type IIa (sPLA-IIa) gene (PLA2G2A) affect sPLA2-IIa level and activity in patients with diabetes mellitus, acute coronary syndrome or recent cardiovascular surgical interventions. Our study examined the effects of PLA2G2A SNPs on sPLA2-IIa levels and activity in patients with stable CHD.

METHODS AND RESULTS:

The study included a total of 396 patients (30% women). Six SNPs of PLA2G2A: rs1774131, rs11573156, rs3753827, rs2236771, rs876018, and rs3767221, sPLA2-IIa level and activity were determined for all patients. Four SNPs (rs1774131, rs11573156, rs3753827, rs3767221) correlated with sPLA2-IIa level but not activity with the strongest correlation observed for rs11573156 (r=0.49, p=3.7·10(-13)). All partial correlations controlling for rs11573156 became insignificant, whereas, the partial correlation of rs11573156 with sPLA2-IIa level controlling for other SNPs remained significant. Only rs11573156 showed association with sPLA2-IIa level in multiple regression analysis. Haplotype CGGGTT was associated with a significantly higher sPLA2-IIa level but not activity compared with all other haplotypes after adjustment for gender, age, diabetes mellitus and statin use (p=0.0023).

CONCLUSIONS:

According to our results the examined SNPs affect the sPLA2-IIa level to a greater extent than its activity in patients with stable CHD. It seems that, the impact of these SNPs on sPLA2-IIa level is caused by their linkage to rs11573156 whose minor alleles were associated with higher sPLA2-IIa level. At the same time haplotype CGGGTT, which includes the minor allele of rs11573156, was the dominant haplotype and was associated with the highest sPLA2-IIa level.

KEYWORDS:

Polymorphisms of PLA2G2A; Stable CHD; sPLA2-IIA activity; sPLA2-IIA level

PMID:
25794429
DOI:
10.1016/j.gene.2015.03.030
[Indexed for MEDLINE]

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