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PLoS One. 2015 Mar 20;10(3):e0118783. doi: 10.1371/journal.pone.0118783. eCollection 2015.

Epibatidine blocks eye-specific segregation in ferret dorsal lateral geniculate nucleus during stage III retinal waves.

Author information

1
Center for Neuroscience, University of California Davis, Davis, California, United States of America.
2
Department of Neurobiology, Physiology, and Behavior, University of California Davis, Davis, California, United States of America.
3
Center for Neuroscience, University of California Davis, Davis, California, United States of America; Department of Neurobiology, Physiology, and Behavior, University of California Davis, Davis, California, United States of America.
4
Center for Neuroscience, University of California Davis, Davis, California, United States of America; Department of Neurobiology, Physiology, and Behavior, University of California Davis, Davis, California, United States of America; Department of Pathology and Laboratory Medicine, University of California Davis, Davis, California, United States of America.

Abstract

The segregation and maintenance of eye-specific inputs in the dorsal lateral geniculate nucleus (dLGN) during early postnatal development requires the patterned spontaneous activity of retinal waves. In contrast to the development of the mouse, ferret eye-specific segregation is not complete at the start of stage III glutamatergic retinal waves, and the remaining overlap is limited to the C/C1 lamina of the dLGN. To investigate the role of patterned spontaneous activity in this late segregation, we disrupted retinal waves pharmacologically for 5 day windows from postnatal day (P) 10 to P25. Multi-electrode array recordings of the retina in vitro reveal that the cholinergic agonist epibatidine disrupts correlated retinal activity during stage III waves. Epibatidine also prevents the segregation of eye-specific inputs in vivo during that period. Our results reveal a novel role for cholinergic influence on stage III retinal waves as an instructive signal for the continued segregation of eye-specific inputs in the ferret dLGN.

PMID:
25794280
PMCID:
PMC4368645
DOI:
10.1371/journal.pone.0118783
[Indexed for MEDLINE]
Free PMC Article

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