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Phys Rev Lett. 2015 Mar 6;114(9):098102. Epub 2015 Mar 2.

Three-dimensional reconstruction of the giant mimivirus particle with an x-ray free-electron laser.

Author information

1
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
2
Génomique & Structurale - IGS - UMR 7256, CNRS, Aix-Marseille Université, Institut de Microbiologie de la Méditerranée, Parc Scientifique de Luminy, Case 934, 13288 Marseille Cedex 9, France.
3
NERSC, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
4
Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
5
LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
6
Attophysics Group, CEA-Saclay, 91191 Gif sur Yvette Cedex, France.
7
Centre for BioImaging Sciences, National University of Singapore, 14 Science Drive 4 Blk S1 A, Singapore 117546, Singapore.
8
The University of Melbourne, Parkville, 3010 Victoria, Australia.
9
University of Hamburg, Notkestrasse 85, 22607 Hamburg, Germany.
10
Max Planck Advanced Study Group, Center for Free Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.
11
J. R. Macdonald Laboratory, Department of Physics, Kansas State University, 116 Cardwell Hall, Manhattan, Kansas 66506, USA.
12
PNSensor GmbH, Römmerstrasse 28, 80803 München, Germany.
13
Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany.
14
Max-Planck-Institut für extraterrestrische Physik, Giessenbachstrasse, 85741 Garching, Germany.
15
European XFEL, Albert-Einstein-Ring 19, 22761 Hamburg, Germany.

Abstract

We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns from an x-ray free-electron laser. Three-dimensional imaging requires the assembly of many two-dimensional patterns into an internally consistent Fourier volume. Since each particle is randomly oriented when exposed to the x-ray pulse, relative orientations have to be retrieved from the diffraction data alone. We achieve this with a modified version of the expand, maximize and compress algorithm and validate our result using new methods.

PMID:
25793853
DOI:
10.1103/PhysRevLett.114.098102
[Indexed for MEDLINE]

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