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ACS Nano. 2015;9(4):3800-13. doi: 10.1021/nn506745r. Epub 2015 Mar 27.

Working together: spatial synchrony in the force and actin dynamics of podosome first neighbors.

Author information

1
†IPBS (Institut de Pharmacologie et de Biologie Structurale), UMR CNRS 5089, 205 route de Narbonne, F-31077 Toulouse, France.
2
‡UPS, Université de Toulouse, F-31400 Toulouse, France.
3
§CNRS, LBCMCP, 118 route de Narbonne, F-31400 Toulouse, France.
4
⊥UPMC, Laboratoire Jean Perrin, FRE 3231 CNRS-UPMC, 4 place Jussieu, F-75005 Paris, France.
5
∥FEMTO-ST, UMR CNRS 6174, Université de Franche Comté, 24 rue de l'Epitaphe, F-25000 Besançon, France.
6
#CNRS, LAAS, 7 avenue du colonel Roche, F-31400 Toulouse, France.
7
∇INSA, Université de Toulouse, F-31400 Toulouse, France.

Abstract

Podosomes are mechanosensitive adhesion cell structures that are capable of applying protrusive forces onto the extracellular environment. We have recently developed a method dedicated to the evaluation of the nanoscale forces that podosomes generate to protrude into the extracellular matrix. It consists in measuring by atomic force microscopy (AFM) the nanometer deformations produced by macrophages on a compliant Formvar membrane and has been called protrusion force microscopy (PFM). Here we perform time-lapse PFM experiments and investigate spatial correlations of force dynamics between podosome pairs. We use an automated procedure based on finite element simulations that extends the analysis of PFM experimental data to take into account podosome architecture and organization. We show that protrusion force varies in a synchronous manner for podosome first neighbors, a result that correlates with phase synchrony of core F-actin temporal oscillations. This dynamic spatial coordination between podosomes suggests a short-range interaction that regulates their mechanical activity.

KEYWORDS:

atomic force microscopy; cell mechanics; macrophage; neighbors; podosome; protrusion force microscopy; synchrony

PMID:
25791988
DOI:
10.1021/nn506745r
[Indexed for MEDLINE]

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