Format

Send to

Choose Destination
Infect Genet Evol. 2015 Jun;32:305-12. doi: 10.1016/j.meegid.2015.03.008. Epub 2015 Mar 17.

Molecular epidemiology and evolutionary dynamics of Echovirus 3 serotype.

Author information

1
Department of Biochemistry & Biotechnology, University of Thessaly, 26, Ploutonos & Aeolou str., Larissa 41 221, Greece. Electronic address: zaxkyr@med.uth.gr.
2
Department of Biochemistry & Biotechnology, University of Thessaly, 26, Ploutonos & Aeolou str., Larissa 41 221, Greece.
3
University of Ioannina, Medical School, Department of Microbiology, Ioannina, Greece.

Abstract

Echovirus 3 (E3) serotype has been related with several neurologic diseases, although it constitutes one of the rarely isolated serotypes, with no report of epidemics in Europe. The aim of the present study was to provide insights into the molecular epidemiology and evolution of this enterovirus serotype, while an E3 strain was isolated from sewage in Greece, four years after the initial isolation of the only reported E3 strain in the same geographical region. Phylogenetic analysis of the complete VP1 genomic region of that E3 strain and of those available in GenBank suggested three main genogroups that were further subdivided into seven subgenogroups. Further evolutionary analysis suggested that VP1 genomic region of E3 was dominated by purifying selection, as the vast majority of genetic diversity presumably occurred through synonymous nucleotide substitutions and the substitution rate for complete and partial VP1 sequences was calculated to be 8.13×10(-3) and 7.72×10(-3) substitutions/site/year respectively. The partial VP1 sequence analysis revealed the composite epidemiology of this serotype, as the strains of the three genogroups presented different epidemiological characteristics.

KEYWORDS:

Echovirus 3; Enteroviruses; Evolution; Molecular epidemiology; VP1

PMID:
25791929
DOI:
10.1016/j.meegid.2015.03.008
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center