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J Am Chem Soc. 2015 Apr 1;137(12):4066-9. doi: 10.1021/jacs.5b00944. Epub 2015 Mar 19.

Chemical methods for the simultaneous quantitation of metabolites and proteins from single cells.

Author information

1
†Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, United States.
2
‡Department of Molecular and Medical Pharmacology, University of California, Los Angeles, California 90095, United States.

Abstract

We describe chemical approaches for integrated metabolic and proteomic assays from single cells. Quantitative assays for intracellular metabolites, including glucose uptake and three other species, are designed as surface-competitive binding assays with fluorescence readouts. This enables integration into a microarray format with functional protein immunoassays, all of which are incorporated into the microchambers of a single-cell barcode chip (SCBC). By using the SCBC, we interrogate the response of human-derived glioblastoma cancer cells to epidermal growth factor receptor inhibition. We report, for the first time, on both the intercellular metabolic heterogeneity as well as the baseline and drug-induced changes in the metabolite-phosphoprotein correlation network.

PMID:
25789560
PMCID:
PMC4883658
DOI:
10.1021/jacs.5b00944
[Indexed for MEDLINE]
Free PMC Article

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