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Biomark Res. 2014 Nov 13;2:20. doi: 10.1186/2050-7771-2-20. eCollection 2014.

Development of TAP, a non-invasive test for qualitative and quantitative measurements of biomarkers from the skin surface.

Author information

1
FibroTx LLC, Mäealuse 4, 12918 Tallinn, Estonia.
2
Dermatology Clinic, Tartu University Hospital, Raja 31, 50407 Tartu, Estonia.
3
FibroTx LLC, Mäealuse 4, 12918 Tallinn, Estonia ; PS! Pharmaconsult, Møllemoseparken 44, 3450 Allerød, Denmark.

Abstract

BACKGROUND:

The skin proteome contains valuable information on skin condition, but also on how skin may evolve in time and may respond to treatments. Despite the potential of measuring regulatory-, effector- and structural proteins in the skin for biomarker applications in clinical dermatology and skin care, convenient diagnostic tools are lacking. The aim of the present study was to develop a highly versatile and non-invasive diagnostic tool for multiplex measurements of protein biomarkers from the surface of skin.

RESULTS:

The Transdermal Analyses Patch (TAP) is a novel molecular diagnostic tool that has been developed to capture biomarkers directly from skin, which are quantitatively analyzed in spot-ELISA assays. Optimisation of protocols for TAP production and biomarker analyses makes TAP measurements highly specific and reproducible. In measurements of interleukin-1α (IL-1α), IL-1 receptor antagonist (IL-1RA) and human β-defensin (hBD-1) from healthy skin, TAP appears far more sensitive than skin lavage-based methods using ELISA. No side-effects were observed using TAP on human skin.

CONCLUSION:

TAP is a practical and valuable new skin diagnostic tool for measuring protein-based biomarkers from skin, which is convenient to use for operators, with minimal burden for patients.

KEYWORDS:

Biomarker; CXCL-1; CXCL-2; Dermatology; Diagnostics; Interleukin 1RA; Interleukin 1α; Skin care; Transdermal analyses patch; β-defensin-1

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