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EMBO Rep. 2015 May;16(5):610-7. doi: 10.15252/embr.201439789. Epub 2015 Mar 17.

Nuclear lamins are not required for lamina-associated domain organization in mouse embryonic stem cells.

Author information

  • 1Division of Gene Regulation, Netherlands Cancer Institute, Amsterdam, the Netherlands.
  • 2Division of Gene Regulation, Netherlands Cancer Institute, Amsterdam, the Netherlands b.v.steensel@nki.nl.

Abstract

In mammals, the nuclear lamina interacts with hundreds of large genomic regions, termed lamina-associated domains (LADs) that are generally in a transcriptionally repressed state. Lamins form the major structural component of the lamina and have been reported to bind DNA and chromatin. Here, we systematically evaluate whether lamins are necessary for the LAD organization in murine embryonic stem cells. Surprisingly, removal of essentially all lamins does not have any detectable effect on the genome-wide interaction pattern of chromatin with emerin, a marker of the inner nuclear membrane. This suggests that other components of the lamina mediate these interactions.

KEYWORDS:

genome‐wide mapping; nuclear architecture; interphase chromosome organization; nuclear lamina

PMID:
25784758
PMCID:
PMC4428043
DOI:
10.15252/embr.201439789
[PubMed - indexed for MEDLINE]
Free PMC Article
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