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J Histochem Cytochem. 1985 Jan;33(1):77-9.

An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide.

Abstract

A rapid, simultaneous double-staining procedure using fluorescein diacetate (FDA) and propidium iodide (PI) is described for use in the determination of cell viability in cell suspension. Air-dried slide preparations can be made from the cell suspensions so that an accurate estimate of the viability of the cells in the original suspension can be made up to 1 week later. Viable cells fluoresce bright green, while nonviable cells are bright red. Furthermore, when FDA-PI staining is compared to trypan blue dye exclusion as a method to determine cell viability, FDA-PI is found to be more consistent over prolonged periods of exposure to the dyes. Therefore, double staining with FDA-PI is a rapid, convenient, and reliable method to determine cell viability.

PMID:
2578146
DOI:
10.1177/33.1.2578146
[Indexed for MEDLINE]

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