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Am J Clin Pathol. 2015 Apr;143(4):500-4. doi: 10.1309/AJCP5BG0KUEOJCVS.

Detection of BRAF mutations on direct smears of thyroid fine-needle aspirates through cell transfer technique.

Author information

1
From the Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis.
2
From the Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis. hhwu@iupui.edu.

Abstract

OBJECTIVES:

To determine the utility of the cell transfer technique (CTT) for BRAF molecular testing on thyroid fine-needle aspiration (FNA) specimens.

METHODS:

Polymerase chain reaction (PCR)-based BRAF molecular testing was performed on tissues obtained through CTT from both air-dried and ethanol-fixed direct smears of thyroid FNA specimens and then compared with the corresponding thyroidectomy formalin-fixed, paraffin-embedded (FFPE) tissues on 30 cases.

RESULTS:

BRAF testing was successfully performed on 29 of 30 air-dried CTT, 27 of 30 ethanol-fixed CTT, and 27 of 30 FFPE tissues. The results exhibited 11, 13, and 13 BRAF mutations and 18, 14, and 14 wild types for the air-dried CTT, the ethanol-fixed CTT, and the FFPE tissues, respectively. The concordance rate was 96% between air-dried and ethanol-fixed CTT tissues, 88% between air-dried CTT and FFPE tissues, and 92% between ethanol-fixed CTT and FFPE tissues.

CONCLUSIONS:

PCR-based BRAF mutational testing can be reliably performed on the direct smears of the thyroid FNA specimens through the application of CTT.

KEYWORDS:

BRAF mutation; Cell transfer; Cytology; Molecular; Thyroid

PMID:
25780001
DOI:
10.1309/AJCP5BG0KUEOJCVS
[Indexed for MEDLINE]
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