Format

Send to

Choose Destination
J Nutr Biochem. 2015 Jun;26(6):585-95. doi: 10.1016/j.jnutbio.2014.12.005. Epub 2015 Feb 4.

Intake of farmed Atlantic salmon fed soybean oil increases hepatic levels of arachidonic acid-derived oxylipins and ceramides in mice.

Author information

1
Department of Biology, University of Copenhagen, Copenhagen, Denmark; National Institute of Nutrition and Seafood Research, Bergen, Norway.
2
Department of Biology, University of Copenhagen, Copenhagen, Denmark; Massachusetts General Hospital, Center for Computational and Integrative Biology, Boston, MA, USA; Obesity and Metabolism Research Unit, United States Department of Agriculture - Agricultural Research Service, Western Human Nutrition Research Center, CA, USA.
3
National Institute of Nutrition and Seafood Research, Bergen, Norway.
4
Massachusetts General Hospital, Center for Computational and Integrative Biology, Boston, MA, USA.
5
Obesity and Metabolism Research Unit, United States Department of Agriculture - Agricultural Research Service, Western Human Nutrition Research Center, CA, USA.
6
Obesity and Metabolism Research Unit, United States Department of Agriculture - Agricultural Research Service, Western Human Nutrition Research Center, CA, USA; Department of Nutrition, University of California, Davis, USA; West Coast Metabolomics Center, University of California, Davis, USA.
7
Department of Biology, University of Copenhagen, Copenhagen, Denmark. Electronic address: kk@bio.ku.dk.
8
Department of Biology, University of Copenhagen, Copenhagen, Denmark; National Institute of Nutrition and Seafood Research, Bergen, Norway. Electronic address: lise.madsen@nifes.no.

Abstract

Introduction of vegetable ingredients in fish feed has affected the fatty acid composition in farmed Atlantic salmon (Salmo salar L). Here we investigated how changes in fish feed affected the metabolism of mice fed diets containing fillets from such farmed salmon. We demonstrate that replacement of fish oil with rapeseed oil or soybean oil in fish feed had distinct spillover effects in mice fed western diets containing the salmon. A reduced ratio of n-3/n-6 polyunsaturated fatty acids in the fish feed, reflected in the salmon, and hence also in the mice diets, led to a selectively increased abundance of arachidonic acid in the phospholipid pool in the livers of the mice. This was accompanied by increased levels of hepatic ceramides and arachidonic acid-derived pro-inflammatory mediators and a reduced abundance of oxylipins derived from eicosapentaenoic acid and docosahexaenoic acid. These changes were associated with increased whole body insulin resistance and hepatic steatosis. Our data suggest that an increased ratio between n-6 and n-3-derived oxylipins may underlie the observed marked metabolic differences between mice fed the different types of farmed salmon. These findings underpin the need for carefully considering the type of oil used for feed production in relation to salmon farming.

KEYWORDS:

Aquaculture; DHA; Diabetes; EPA; Lipidomics; Obesity

PMID:
25776459
DOI:
10.1016/j.jnutbio.2014.12.005
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center