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J Nutr Biochem. 2015 Jun;26(6):661-6. doi: 10.1016/j.jnutbio.2015.01.004. Epub 2015 Mar 5.

Myrosinase-treated glucoerucin is a potent inducer of the Nrf2 target gene heme oxygenase 1--studies in cultured HT-29 cells and mice.

Author information

1
Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany. Electronic address: wagner@molecularnutrition.uni-kiel.de.
2
Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany. Electronic address: sturm@foodsci.uni-kiel.de.
3
Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany. Electronic address: piegholdt@foodsci.uni-kiel.de.
4
Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany. Electronic address: i.wolf@uke.de.
5
Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany. Electronic address: esatbeyoglu@foodsci.uni-kiel.de.
6
Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di ricerca per le colture industriali, Via Di Corticella 133, Bologna 40128, Italy. Electronic address: ginarosalinda.denicola@entecra.it.
7
Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di ricerca per le colture industriali, Via Di Corticella 133, Bologna 40128, Italy. Electronic address: renato.iori@entecra.it.
8
Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany. Electronic address: rimbach@foodsci.uni-kiel.de.

Abstract

In this study, the effect of myrosinase-treated glucoerucin (GER+MYR), which releases the isothiocyanate (ITC) erucin, on heme oxygenase 1 (HO-1) gene expression and Nrf2 signaling was investigated in vitro in cultured cells and in vivo in mice. Treatment of HT-29 cells with GER+MYR resulted in a significant increase in the mRNA and protein levels of nuclear Nrf2 and HO-1. GER+MYR was more potent at enhancing the nuclear Nrf2 levels than were the following myrosinase-treated glucosinolates: sinigrin, glucoraphanin and gluconasturtiin, which are the precursors of allyl-ITC, R-sulforaphane and 2-phenylethyl ITC, respectively. GER+MYR also significantly induced HO-1 gene expression in the mouse intestinal mucosae and liver but not in the brain. Mechanistic studies suggest that GER+MYR induces Nrf2 via ERK1/2-, p38- and JNK-dependent signal transduction pathways. The GER+MYR-mediated increase in HO-1 expression is primarily attributable to p38 signaling.

KEYWORDS:

Glucoerucin; Glucosinolates; Heme oxygenase 1; MAPK; Myrosinase; Nrf2

PMID:
25776458
DOI:
10.1016/j.jnutbio.2015.01.004
[Indexed for MEDLINE]

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