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Nat Methods. 2015 May;12(5):404-6. doi: 10.1038/nmeth.3323. Epub 2015 Mar 16.

CIDRE: an illumination-correction method for optical microscopy.

Author information

1
Scientific Center for Optical and Electron Microscopy, ETH Zürich, Zurich, Switzerland.
2
Computer Vision Laboratory, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
3
Advanced Research Center on Electronic Systems, University of Bologna, Bologna, Italy.
4
1] Advanced Research Center on Electronic Systems, University of Bologna, Bologna, Italy. [2] Department of Computer Science and Engineering, University of Bologna, Bologna, Italy.
5
1] Institute of Biochemistry, ETH Zürich, Zurich, Switzerland. [2] Synthetic and System Biology Unit, Biological Research Center, Szeged, Hungary. [3] Institute for Molecular Medicine Finland, University of Helsinki, Helsinki, Finland.

Abstract

Uneven illumination affects every image acquired by a microscope. It is often overlooked, but it can introduce considerable bias to image measurements. The most reliable correction methods require special reference images, and retrospective alternatives do not fully model the correction process. Our approach overcomes these issues for most optical microscopy applications without the need for reference images.

PMID:
25775044
DOI:
10.1038/nmeth.3323
[Indexed for MEDLINE]

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