Format

Send to

Choose Destination
Nat Methods. 2015 May;12(5):411-4. doi: 10.1038/nmeth.3319. Epub 2015 Mar 16.

Direct visualization of newly synthesized target proteins in situ.

Author information

1
Synaptic Plasticity Department, Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
2
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, USA.

Abstract

Protein synthesis is a dynamic process that tunes the cellular proteome in response to internal and external demands. Metabolic labeling approaches identify the general proteomic response but cannot visualize specific newly synthesized proteins within cells. Here we describe a technique that couples noncanonical amino acid tagging or puromycylation with the proximity ligation assay to visualize specific newly synthesized proteins and monitor their origin, redistribution and turnover in situ.

PMID:
25775042
PMCID:
PMC4414919
DOI:
10.1038/nmeth.3319
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center