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Nat Prod Res. 2016;30(4):460-3. doi: 10.1080/14786419.2015.1020489. Epub 2015 Mar 16.

Antiproliferative effect of isolated isoquinoline alkaloid from Mucuna pruriens seeds in hepatic carcinoma cells.

Author information

1
a Department of Pharmaceutical Sciences , Babasaheb Bhimrao Ambedkar University , Vidya Vihar, Raebareli Road, Lucknow 226025 , India.
2
b Sanjay Gandhi Post-Graduate Institute of Medical Sciences Campus, Centre of Biomedical Research , Raebareli Road, Lucknow 226014 , Uttar Pradesh , India.
3
c Department of Pharmaceutical Technology , Jadavpur University , Kolkata 700032 , India.

Abstract

The present study was undertaken to investigate the antiproliferative action of isolated M1 (6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid) from Mucuna pruriens seeds using human hepatic carcinoma cell line (Huh-7 cells). Initially, docking studies was performed to find out the binding affinities of M1 to caspase-3 and 8 enzymes. Later, cytotoxic action of M1 was measured by cell growth inhibition (MTT), followed by caspase-3 and 8 enzymes assay colorimetrically. Our results collectively suggested that M1 had strong binding affinity to caspase-8 in molecular modelling. M1 possessed antiproliferative activity on Huh-7 cells (EC50 = 13.97 μM) and also inhibited the action of caspase-8 enzyme, signified process of apoptosis. M1 was active against Huh-7 cells that may be useful for future hepatic cancer treatment.

KEYWORDS:

Huh-7 cells; caspase-3 and 8; isoquinoline alkaloid; molecular docking

PMID:
25774560
DOI:
10.1080/14786419.2015.1020489
[Indexed for MEDLINE]

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