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Acta Neuropsychiatr. 2015 Oct;27(5):307-11. doi: 10.1017/neu.2015.10. Epub 2015 Mar 16.

Dietary magnesium deficiency affects gut microbiota and anxiety-like behaviour in C57BL/6N mice.

Author information

1
1Section of Experimental Animal Models,Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences,University of Copenhagen,Frederiksberg C,Denmark.
2
2Translational Neuropsychiatry Unit,Department of Clinical Medicine,Aarhus University,Risskov,Denmark.
3
3Department of Food Science, Faculty of Science,University of Copenhagen,Frederiksberg C,Denmark.

Abstract

OBJECTIVE:

Magnesium deficiency has been associated with anxiety in humans, and rodent studies have demonstrated the gut microbiota to impact behaviour.

METHODS:

We investigated the impact of 6 weeks of dietary magnesium deficiency on gut microbiota composition and anxiety-like behaviour and whether there was a link between the two. A total of 20 C57BL/6 mice, fed either a standard diet or a magnesium-deficient diet for 6 weeks, were tested using the light-dark box anxiety test. Gut microbiota composition was analysed by denaturation gradient gel electrophoresis.

RESULTS:

We demonstrated that the gut microbiota composition correlated significantly with the behaviour of dietary unchallenged mice. A magnesium-deficient diet altered the gut microbiota, and was associated with altered anxiety-like behaviour, measured by decreased latency to enter the light box.

CONCLUSION:

Magnesium deficiency altered behavior. The duration of magnesium deficiency is suggested to influence behaviour in the evaluated test.

KEYWORDS:

animal models; anxiety; gut microbiota; magnesium

PMID:
25773775
DOI:
10.1017/neu.2015.10
[Indexed for MEDLINE]

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