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Environ Mol Mutagen. 2015 Jul;56(6):491-504. doi: 10.1002/em.21944. Epub 2015 Mar 12.

DNA damage foci: Meaning and significance.

Author information

1
Public Health England, Centre for Radiation, Chemical and Environmental Hazards, Chilton, United Kingdom.
2
Department of Radiotherapy, Laboratory of Radiation Biology and Experimental Radiation Oncology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
3
Department of Cellular Pathology, Oxford University Hospitals, Headley Way, Headington, Oxford, United Kingdom.

Abstract

The discovery of DNA damage response proteins such as γH2AX, ATM, 53BP1, RAD51, and the MRE11/RAD50/NBS1 complex, that accumulate and/or are modified in the vicinity of a chromosomal DNA double-strand break to form microscopically visible, subnuclear foci, has revolutionized the detection of these lesions and has enabled studies of the cellular machinery that contributes to their repair. Double-strand breaks are induced directly by a number of physical and chemical agents, including ionizing radiation and radiomimetic drugs, but can also arise as secondary lesions during replication and DNA repair following exposure to a wide range of genotoxins. Here we aim to review the biological meaning and significance of DNA damage foci, looking specifically at a range of different settings in which such markers of DNA damage and repair are being studied and interpreted.

KEYWORDS:

53BP1; DNA double-strand break; genotoxicity; ionizing radiation; γH2AX

PMID:
25773265
DOI:
10.1002/em.21944
[Indexed for MEDLINE]

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