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Microbiologyopen. 2015 Jun;4(3):465-74. doi: 10.1002/mbo3.252. Epub 2015 Mar 15.

A highly acid-resistant novel strain of Lactobacillus johnsonii No. 1088 has antibacterial activity, including that against Helicobacter pylori, and inhibits gastrin-mediated acid production in mice.

Author information

1
Laboratory for Infectious Diseases, Tokai University School of Medicine, Isehara, Kanagawa, 259-1103, Japan.
2
Snowden Co., Ltd., Chiyoda-ku, Tokyo, 101-0032, Japan.

Abstract

A novel strain of Lactobacillus johnsonii No. 1088 was isolated from the gastric juice of a healthy Japanese male volunteer, and characterized for its effectiveness in the stomach environment. Lactobacillus johnsonii No. 1088 was found to have the strongest acid resistance among several lactobacilli examined (>10% of cells survived at pH 1.0 after 2 h), and such a high acid resistance property was a specific characteristic of this strain of L. johnsonii. When cultured with various virulent bacteria, L. johnsonii No. 1088 inhibited the growth of Helicobacter pylori, Escherichia coli O-157, Salmonella Typhimurium, and Clostridium difficile, in which case its effectiveness was more potent than that of a type strain of L. johnsonii, JCM2012. In addition to its effect in vitro, L. johnsonii No. 1088 inhibited the growth of H. pylori in human intestinal microbiota-associated mice in both its live and lyophilized forms. Moreover, L. johnsonii No. 1088 suppressed gastric acid secretion in mice via decreasing the number of gastrin-positive cells in the stomach. These results taken together suggest that L. johnsonii No. 1088 is a unique lactobacillus having properties beneficial for supporting H. pylori eradication by triple therapy including the use of a proton pump inhibitor (PPI) and also for prophylaxis of gastroesophageal reflux disease possibly caused after H. pylori eradication as a side effect of PPI.

KEYWORDS:

Gastroesophageal reflux disease; Helicobacter pylori; Lactobacillus johnsonii; lactic acid bacteria; probiotics

PMID:
25771812
PMCID:
PMC4475388
DOI:
10.1002/mbo3.252
[Indexed for MEDLINE]
Free PMC Article

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