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Nutrition. 2015 Apr;31(4):570-7. doi: 10.1016/j.nut.2014.06.001. Epub 2014 Jun 23.

Synergistic anti-tumor effects of melatonin and PUFAs from walnuts in a murine mammary adenocarcinoma model.

Author information

1
Instituto de Biología Celular-Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
2
Instituto Multidisciplinario de Biología Vegetal (IMBIV-CONICET), Cátedra de Química Orgánica. FCEF y N. UNC, Argentina.
3
Instituto de Investigaciones en Ciencias de la Salud de Córdoba (INICSA-CONICET) and Cátedra de Biología Celular, Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba (FCM-UNC), Ciudad Universitaria, Córdoba, Argentina.
4
UND Life Sciences, Shaker Heights, Ohio, United States; Jawaharlal Nehru Technological University, Kakinada, India; Bio-Science Research Centre, Gayatri Vidya Parishad College of Engineering, Visakhapatnam, India.
5
Instituto de Investigaciones en Ciencias de la Salud de Córdoba (INICSA-CONICET) and Cátedra de Biología Celular, Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba (FCM-UNC), Ciudad Universitaria, Córdoba, Argentina. Electronic address: Epasqual@cmefcm.uncor.edu.

Abstract

OBJECTIVE:

The aim of this study was to determine the effects of some polyunsaturated fatty acids plus phytomelatonin from walnuts in the development of mammary gland adenocarcinoma.

METHODS:

BALB/c mice were fed a semisynthetic diet supplemented with either 6% walnut oil and 8% walnut flour containing phytomelatonin (walnut diet: WD); or 6% corn oil plus commercial melatonin (melatonin diet: MD), or the control group (CD), which received only 6% of corn oil. Membrane fatty acids of tumor cells (TCs) were analyzed by gas liquid chromatography, cyclooxygenase (COX) and lipoxygenase (LOX) derivatives, and plasma melatonin by high-performance liquid chromatography; apoptosis and tumor-infiltrating lymphocytes by flow cytometry.

RESULTS:

TCs from the MD and WD mice showed significant decreases in linoleic acid compared with the CD group (P < 0.05). Significantly lower levels of LOX-[13(S)-HODE] were found in TCs from the MD and WD group than in CD (P < 0.0001). COX-[12(S)-HHT] was lower and 12 LOX-[12(S)-HETE] was higher in TCs from the MD group than form the WD and CD arms (P < 0.05). Plasma melatonin, apoptosis, tumor infiltration, and survival time were significantly lower in CD mice than in MD and WD mice (P < 0.05).

CONCLUSIONS:

This study shows that melatonin, along with polyunsaturated fatty acids, exerts a selective inhibition of some COX and LOX activities and has a synergistic anti-tumor effect on a mammary gland adenocarcinoma model.

KEYWORDS:

Cancer; Diet; Eicosanoids; PUFAs; Phytomelatonin

PMID:
25770319
DOI:
10.1016/j.nut.2014.06.001
[Indexed for MEDLINE]

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