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Microb Pathog. 2015 Apr;81:6-15. doi: 10.1016/j.micpath.2015.03.007. Epub 2015 Mar 11.

Deciphering the protein interaction in adhesion of Francisella tularensis subsp. holarctica to the endothelial cells.

Author information

1
Laboratory of Biomedical Microbiology and Immunology, Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia.
2
Institute of Neuroimmunology, Slovak Academy of Sciences, 845 10 Bratislava, Slovakia.
3
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, H-1581 Budapest, Hungary.
4
Bioinformatics & Medical Informatics Team, Biomedical Research Foundation, Academy of Athens, Athens 11527, Greece.
5
Laboratory of Biomedical Microbiology and Immunology, Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia; Institute of Neuroimmunology, Slovak Academy of Sciences, 845 10 Bratislava, Slovakia. Electronic address: bhidemangesh@gmail.com.

Abstract

Extracellular form of Francisella is able to cross various cell barriers and invade multiple organs, such as skin, liver, lung and central nervous system. Transient adhesion of Francisella to endothelial cells may trigger the process of translocation. In this report, we showed that Francisella tularensis subsp. holarctica (Fth) is able to adhere to the endothelial cells, while ICAM-1 may serve as an adhesion molecule for Fth. Pull down and affinity ligand binding assays indicated that the PilE4 could be the probable ligand for ICAM-1. Further deciphering of this ligand:receptor interaction revealed that PilE4 interacts with Ig-like C2-type 1 domain of ICAM-1. To corroborate the role of PilE4 and ICAM-1 interaction in adhesion of extracellular form of Fth to endothelial cells, ICAM-1 was blocked with monoclonal anti-ICAM-1 antibody prior to the incubation with Fth and numbers of adherent bacteria were counted. Blocking of the ICAM-1 significantly reduced (500-fold, P < 0.05) number of adherent Fth compared to unblocked cells. PilE4:ICAM-1 interaction unfolded here may provide a new perspective on molecules involved in the adhesion of extracellular form of Francisella to endothelial cells and probably its translocation across endothelial barriers.

KEYWORDS:

Endothelial cells; Francisella; ICAM-1; Type IV pili

PMID:
25769821
DOI:
10.1016/j.micpath.2015.03.007
[Indexed for MEDLINE]

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