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Transplant Proc. 2015 Mar;47(2):536-44. doi: 10.1016/j.transproceed.2014.11.031.

Coconut water solutions for the preservation of spleen, ovary, and skin autotransplants in rats.

Author information

1
Surgery Department of Minas Gerais Federal University, Belo Horizonte, Brazil. Electronic address: jorgemiguels@ig.com.br.
2
Surgery Department of Minas Gerais Federal University, Belo Horizonte, Brazil.
3
Clinical Pathology Department of Minas Gerais University, Belo Horizonte, Brazil.
4
Pharmacology Department of Minas Gerais University, Belo Horizonte, Brazil.
5
Pathology Department of Minas Gerais, Belo Horizonte, Brazil.

Abstract

BACKGROUND:

The purpose of this study was to evaluate the efficacy of coconut water in the preservation of spleen, ovary, and skin autotransplantations in rats.

METHODS:

Fifty female Wistar rats were divided randomly into 5 groups on the basis of the following tissue graft preservation solutions: group 1, lactated Ringer's; group 2, Belzer's solution; group 3, mature coconut water; group 4, green coconut water; and group 5, modified green coconut water. In group 5, the green coconut water solution was modified to obtain the same electrolyte composition as Belzer's solution. The spleen, ovaries, and a skin fragment were removed from each animal, stored for 6 hours in one of the solutions, and then re-implanted. The recoveries of tissue functions were assessed 90 days after surgery by means of spleen scintigraphy and blood tests. The implanted tissues were collected for histological analyses.

RESULTS:

Higher immunoglobulin G levels were observed in the animals of group 5 than in the animals of group 1. Differences in follicle-stimulating hormone levels were observed between groups 1 and 2 (P < .001), between groups 4 and 2 (P = .03), and between groups 5 and 2 (P = .01). The spleen scintigraphy results did not differ among the groups. The ovarian tissue was better preserved in the mature coconut water group (P < .007).

CONCLUSIONS:

Solutions containing coconut water allowed for the preservation of the spleen, ovaries, and skin for 6 hours, and the normal functions of these tissues were maintained in rats.

[Indexed for MEDLINE]

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