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Nat Commun. 2015 Mar 13;6:6610. doi: 10.1038/ncomms7610.

Sumoylation controls the timing of Tup1-mediated transcriptional deactivation.

Author information

1
1] Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, 1117 Fairchild Center, New York, New York 10027, USA [2] Faculty of Information Science &Technology, Multimedia University, Jalan Ayer Keroh Lama, 75450 Bukit Beruang, Melaka, Malaysia.
2
Department of Biology, York University, Toronto, Ontario, Canada M3J 1P3.
3
Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, 1117 Fairchild Center, New York, New York 10027, USA.

Abstract

The small ubiquitin-like modifier (SUMO) is implicated in various cellular activities, including transcriptional regulation. We previously showed that the yeast activator Gcn4 becomes sumoylated during activation, facilitating its eventual promoter eviction and transcriptional shut off. Here we show that the corepressor Tup1 is sumoylated, at two specific lysines, under various stress conditions. Mutation of these sites has no effect on Tup1 recruitment or RNAP II promoter occupancy immediately following induction. However, Tup1 levels subsequently decrease, while RNAP II and transcription increase in Tup1 mutant cells. Consistent with this, a Tup1 mutant displaying increased sumoylation led to reduced transcription. We also show that coordinated sumoylation of Gcn4 and Tup1 enhances Gcn4 promoter eviction and that multiple Tup1-interacting proteins become sumoylated after stress. Together, our studies provide evidence that coordinated sumoylation of Gcn4, Tup1 and likely other factors dampens activated transcription by stabilizing Tup1 binding and stimulating Gcn4 and RNAP II removal.

PMID:
25766875
PMCID:
PMC4360881
DOI:
10.1038/ncomms7610
[Indexed for MEDLINE]
Free PMC Article

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