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Dev Comp Immunol. 2015 Aug;51(2):287-97. doi: 10.1016/j.dci.2015.02.019. Epub 2015 Mar 9.

The early stages of the immune response of the European abalone Haliotis tuberculata to a Vibrio harveyi infection.

Author information

1
UMR 6539-LEMAR (Laboratoire des Sciences de l'Environnement Marin), IUEM (Institut Universitaire Européen de la Mer), Université de Bretagne Occidentale (UBO), CNRS, IRD, Ifremer, Technopôle Brest Iroise, 29280 Plouzané, France. Electronic address: marion.cardinaud@gmail.com.
2
School of Marine and Atmospheric Sciences, Stonybrook University, Stony Brook, NY 11794-5000 USA.
3
France Haliotis, Kerazan, Lilia, 29880 Plouguerneau, France.
4
UMR 6539-LEMAR (Laboratoire des Sciences de l'Environnement Marin), IUEM (Institut Universitaire Européen de la Mer), Université de Bretagne Occidentale (UBO), CNRS, IRD, Ifremer, Technopôle Brest Iroise, 29280 Plouzané, France.
5
UMR 6539-LEMAR (Laboratoire des Sciences de l'Environnement Marin), IUEM (Institut Universitaire Européen de la Mer), Université de Bretagne Occidentale (UBO), CNRS, IRD, Ifremer, Technopôle Brest Iroise, 29280 Plouzané, France. Electronic address: christine.paillard@univ-brest.fr.

Abstract

Vibrio harveyi is a marine bacterial pathogen responsible for episodic abalone mortalities in France, Japan and Australia. In the European abalone, V. harveyi invades the circulatory system in a few hours after exposure and is lethal after 2 days of infection. In this study, we investigated the responses of European abalone immune cells over the first 24 h of infection. Results revealed an initial induction of immune gene expression including Rel/NF-kB, Mpeg and Clathrin. It is rapidly followed by a significant immuno-suppression characterized by reduced cellular hemocyte parameters, immune response gene expressions and enzymatic activities. Interestingly, Ferritin was overexpressed after 24 h of infection suggesting that abalone attempt to counter V. harveyi infection using soluble effectors. Immune function alteration was positively correlated with V. harveyi concentration. This study provides the evidence that V. harveyi has a hemolytic activity and an immuno-suppressive effect in the European abalone.

KEYWORDS:

Abalone immune response; Flow cytometry; Gene expression; Pathogen quantification; Vibrio harveyi

PMID:
25766281
DOI:
10.1016/j.dci.2015.02.019
[Indexed for MEDLINE]

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