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PLoS One. 2015 Mar 12;10(3):e0119347. doi: 10.1371/journal.pone.0119347. eCollection 2015.

Condensin HEAT subunits required for DNA repair, kinetochore/centromere function and ploidy maintenance in fission yeast.

Author information

1
Okinawa Institute of Science and Technology Graduate University, Onna-son, Okinawa, Japan; Department of Genetics, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.
2
Okinawa Institute of Science and Technology Graduate University, Onna-son, Okinawa, Japan.

Abstract

Condensin, a central player in eukaryotic chromosomal dynamics, contains five evolutionarily-conserved subunits. Two SMC (structural maintenance of chromosomes) subunits contain ATPase, hinge, and coiled-coil domains. One non-SMC subunit is similar to bacterial kleisin, and two other non-SMC subunits contain HEAT (similar to armadillo) repeats. Here we report isolation and characterization of 21 fission yeast (Schizosaccharomyces pombe) mutants for three non-SMC subunits, created using error-prone mutagenesis that resulted in single-amino acid substitutions. Beside condensation, segregation, and DNA repair defects, similar to those observed in previously isolated SMC and cnd2 mutants, novel phenotypes were observed for mutants of HEAT-repeats containing Cnd1 and Cnd3 subunits. cnd3-L269P is hypersensitive to the microtubule poison, thiabendazole, revealing defects in kinetochore/centromere and spindle assembly checkpoints. Three cnd1 and three cnd3 mutants increased cell size and doubled DNA content, thereby eliminating the haploid state. Five of these mutations reside in helix B of HEAT repeats. Two non-SMC condensin subunits, Cnd1 and Cnd3, are thus implicated in ploidy maintenance.

PMID:
25764183
PMCID:
PMC4357468
DOI:
10.1371/journal.pone.0119347
[Indexed for MEDLINE]
Free PMC Article

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