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J Orthop Res. 2015 Aug;33(8):1180-7. doi: 10.1002/jor.22874. Epub 2015 Apr 10.

T2* relaxometry and volume predict semi-quantitative histological scoring of an ACL bridge-enhanced primary repair in a porcine model.

Author information

1
Department of Orthopaedics, Warren Alpert Medical School, Brown University/Rhode Island Hospital, Suite 404, 1 Hoppin Street, Providence, Rhode Island.
2
School of Engineering, Brown University, Providence, Rhode Island.
3
Department of Orthopaedic Surgery, Children's Hospital Boston, Boston, Massachusetts.
4
Department of Neuroscience, Division of Biology and Medicine, Brown University, Providence, Rhode Island.

Abstract

Magnetic resonance imaging (MRI) variables, such as T2* and volume, can predict the healing ligament structural properties. How these MR variables relate to semi-quantitative histology of the healing ACL is yet unknown. We hypothesized that T2* and volume would predict the histological scoring of a healing ACL. Yucatan minipigs underwent ACL transection and received bridge-enhanced ACL repair or no treatment. The surgical legs were harvested after 52 weeks and imaged using a high resolution 2-echo sequence. For each ligament, the volume and median T2* values were determined. The ACL specimens were then histologically analyzed using the advanced Ligament Maturity Index (LMI). The T2* of the healing ligaments significantly predicted the total LMI score as well as the cell, collagen and vessel sub-scores; R(2)  = 0.78, 0.67, 0.65, and 0.60, respectively (p ≤ 0.001). The ligament volume also predicted the total LMI score, cell, and collagen sub-scores; R(2)  = 0.39, 0.33, 0.37, and 0.60, respectively (p ≤ 0.001). A lower ligament T2* or a higher volume was associated with higher histological scores of the healing ligaments. This study provides a critical step in the development of a non-invasive method to evaluate ligament healing on a microscopic scale.

KEYWORDS:

ACL; MRI; healing; histology; ligament

PMID:
25764143
PMCID:
PMC4497917
DOI:
10.1002/jor.22874
[Indexed for MEDLINE]
Free PMC Article

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