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Transcription. 2014;5(1):e27526. doi: 10.4161/trns.27526.

Contributions of in vitro transcription to the understanding of human RNA polymerase III transcription.

Author information

1
a INSERM U869; University of Bordeaux; Institut Européen de Chimie et Biologie (IECB); 33607 Pessac, France.

Abstract

Human RNA polymerase III transcribes small untranslated RNAs that contribute to the regulation of essential cellular processes, including transcription, RNA processing and translation. Analysis of this transcription system by in vitro transcription techniques has largely contributed to the discovery of its transcription factors and to the understanding of the regulation of human RNA polymerase III transcription. Here we review some of the key steps that led to the identification of transcription factors and to the definition of minimal promoter sequences for human RNA polymerase III transcription.

KEYWORDS:

Human RNA polymerase III; PTF; SNAPC; TFIIIA; TFIIIB; TFIIIC; activated transcription; basal transcription; enhancer; in vitro; promoter

PMID:
25764111
PMCID:
PMC4214236
DOI:
10.4161/trns.27526
[Indexed for MEDLINE]
Free PMC Article

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