Format

Send to

Choose Destination
Braz J Microbiol. 2015 Mar 4;45(4):1333-9. eCollection 2014.

Cultivable endophytic bacteria from leaf bases of Agave tequilana and their role as plant growth promoters.

Author information

1
Instituto de Ingeniería Universidad Autónoma de Baja California Campus MexicaliMexicali México Instituto de Ingeniería, Universidad Autónoma de Baja California, Campus Mexicali, Mexicali, México.
2
Departamento de Química Instituto de Ciencias Exactas y Terrestres Universidad Autónoma de Guadalajara Jalisco México Departamento de Química, Instituto de Ciencias Exactas y Terrestres, Universidad Autónoma de Guadalajara, Jalisco, México.
3
Colegio de Postgraduados Carretera México-Texcoco Texcoco México Colegio de Postgraduados Carretera México-Texcoco, Texcoco, México.
4
El Colegio de la Frontera Sur A.C. Unidad Campeche LermaCampeche México El Colegio de la Frontera Sur A.C. Unidad Campeche, Lerma, Campeche, México.
5
Departamento de Ingeniería Celular y Biocatálisis Instituto de Biotecnología Universidad Nacional Autónoma de México CuernavacaMorelos México Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.

Abstract

Agave tequilana Weber var. 'Azul' is grown for the production of tequila, inulin and syrup. Diverse bacteria inhabit plant tissues and play a crucial role for plant health and growth. In this study culturable endophytic bacteria were extracted from leaf bases of 100 healthy Agave tequilana plants. In plant tissue bacteria occurred at mean population densities of 3 million CFU/g of fresh plant tissue. Three hundred endophytic strains were isolated and 16s rDNA sequences grouped the bacteria into eight different taxa that shared high homology with other known sequences. Bacterial endophytes were identified as Acinectobacter sp., A. baumanii, A. bereziniae, Cronobacter sakazakii, Enterobacter hormaechei, Bacillus sp. Klebsiella oxytoca, Pseudomonas sp., Enterococcus casseliflavus, Leuconostoc mesenteroides subsp. mesenteroides and Gluconobacter oxydans. Isolates were confirmed to be plant growth promoting bacteria (PGPB) by their capacities for nitrogen fixation, auxin production, phosphate solubilization, or antagonism against Fusarium oxysporum AC132. E. casseliflavus JM47 and K. oxytoca JM26 secreted the highest concentrations of IAA. The endophyte Acinectobacter sp. JM58 exhibited the maximum values for nitrogen fixation and phosphate solubilization index (PSI). Inhibition of fungi was found in Pseudomonas sp. JM9p and K. oxytoca JM26. Bacterial endophytes show promise for use as bio-inoculants for agave cultivation. Use of endophytes to enhance cultivation of agave may be particularly important for plants produced by micropropagation techniques, where native endophytes may have been lost.

KEYWORDS:

Agave tequilana; Fusarium oxysporum; endophytic; pathogen inhibition

PMID:
25763038
PMCID:
PMC4323307
DOI:
10.1590/s1517-83822014000400025
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center