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J Clin Microbiol. 2015 Jun;53(6):1806-11. doi: 10.1128/JCM.03505-14. Epub 2015 Mar 11.

The Resistant-Population Cutoff (RCOFF): a New Concept for Improved Characterization of Antimicrobial Susceptibility Patterns of Non-Wild-Type Bacterial Populations.

Author information

1
Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland.
2
Unité des Agents Antibactériens, Institut Pasteur, Paris, France.
3
Epidemiology, Biostatistics and Prevention Institute, University of Zurich, Zurich, Switzerland.
4
Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland boettger@imm.uzh.ch.

Abstract

This study aimed to determine resistant-population cutoffs (RCOFFs) to allow for improved characterization of antimicrobial susceptibility patterns in bacterial populations. RCOFFs can complement epidemiological cutoff (ECOFF)-based settings of clinical breakpoints (CBPs) by systematically describing the correlation between non-wild-type and wild-type populations. We illustrate this concept by describing three paradigmatic examples of wild-type and non-wild-type Escherichia coli populations from our clinical strain database of disk diffusion diameters. The statistical determination of RCOFFs and ECOFFs and their standardized applications in antimicrobial susceptibility testing (AST) facilitates the assignment of isolates to wild-type or non-wild-type populations. This should improve the correlation of in vitro AST data and distinct antibiotic resistance mechanisms with clinical outcome facilitating the setting and validation of CBPs.

PMID:
25762769
PMCID:
PMC4432042
DOI:
10.1128/JCM.03505-14
[Indexed for MEDLINE]
Free PMC Article

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