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Neuron. 1989 Aug;3(2):257-65.

Cycling of actin assembly in synaptosomes and neurotransmitter release.

Author information

1
Department of Biochemistry, Colorado State University, Fort Collins 80523.

Abstract

We have investigated the regulation of actin assembly in whole mouse brain synaptosomes and how that regulation modulates neurotransmitter release. During a 30 s depolarization with high K+, filamentous actin (F-actin) levels, monitored by staining with rhodamine phalloidin, increase dramatically (up to 300% in 3 s), decrease, and increase once again. This F-actin cycling is regulated by pathways both dependent and independent of Ca2+ influx and is markedly affected by exposing synaptosomes to Li+, tetrodotoxin, and diacylglycerol. Measurement of [3H]norepinephrine release from synaptosomes containing entrapped agents that modulate actin assembly (DNAase I or phalloidin) indicates that actin depolymerization is necessary for normal release and that repolymerization limits release.

PMID:
2576215
DOI:
10.1016/0896-6273(89)90039-1
[Indexed for MEDLINE]

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