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Sci Rep. 2015 Mar 11;5:9005. doi: 10.1038/srep09005.

A monomer-trimer model supports intermittent glucagon fibril growth.

Author information

1
Harvard University, Department of Physics, 17 Oxford Street, Cambridge, MA 02138, USA.
2
Copenhagen University, Niels Bohr Institute, CMOL, Blegdamsvej 17, DK-2100 Copenhagen, Denmark.
3
1] Copenhagen University, Niels Bohr Institute, CMOL, Blegdamsvej 17, DK-2100 Copenhagen, Denmark [2] Interdisciplinary Nanoscience Center (iNANO), Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 14, DK-8000 Aarhus C, Denmark.

Abstract

We investigate in vitro fibrillation kinetics of the hormone peptide glucagon at various concentrations using confocal microscopy and determine the glucagon fibril persistence length 60μm. At all concentrations we observe that periods of individual fibril growth are interrupted by periods of stasis. The growth probability is large at high and low concentrations and is reduced for intermediate glucagon concentrations. To explain this behavior we propose a simple model, where fibrils come in two forms, one built entirely from glucagon monomers and one entirely from glucagon trimers. The opposite building blocks act as fibril growth blockers, and this generic model reproduces experimental behavior well.

PMID:
25758791
PMCID:
PMC4355668
DOI:
10.1038/srep09005
[Indexed for MEDLINE]
Free PMC Article

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