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Ann N Y Acad Sci. 2015 Sep;1352:13-9. doi: 10.1111/nyas.12660. Epub 2015 Mar 10.

Designing optogenetically controlled RNA for regulating biological systems.

Author information

1
Department of Pharmacology, Weill Cornell Medical College, New York, New York.

Abstract

Light-responsive proteins have been used in the field of optogenetics to control cellular functions. However, surprisingly, analogous approaches to regulate and alter the functions of RNA molecules by light remain underdeveloped. RNA aptamers and RNA devices can perform diverse intracellular functions and are important tools in synthetic biology. This report explores the challenges of and potential strategies for engineering light regulation into functional RNAs in cells. We discuss approaches for using existing light-regulated proteins and small molecules to control RNA function in living cells. In addition, applications of light-regulated RNAs for synthetic biology and for studying functions of endogenously expressed RNAs are discussed.

KEYWORDS:

RNA aptamer; genetic encoding; optogenetics; photoswitching; synthetic biology

PMID:
25758022
PMCID:
PMC4565785
DOI:
10.1111/nyas.12660
[Indexed for MEDLINE]
Free PMC Article

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