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Nat Commun. 2015 Mar 11;6:6492. doi: 10.1038/ncomms7492.

Organelles maintain spindle position in plant meiosis.

Author information

1
Department of Biochemistry, University of Otago, PO Box 56, Dunedin 9054, New Zealand.
2
Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center of Plant Biology, PO Box 7080, Uppsala SE-75007, Sweden.
3
Department of Biology, University of Leicester, Leicester LE1 7RH, UK.

Abstract

Accurate positioning of spindles is a critical aspect of cell division as it ensures that each daughter cell contains a single nucleus. In many flowering plants, two meiotic chromosome separations occur without intervening cytokinesis, resulting in two spindles in one cell during the second division. Here we report a detailed examination of two mutants, jason (jas) and parallel spindle1 (ps1), in which disturbed spindle position during male meiosis II results in the incorporation of previously separated chromosome groups into a single cell. Our study reveals that an organelle band provides a physical barrier between the two spindles. The loss of a single protein, JAS, from this organelle band leads to its disruption and a random movement of the spindles. JAS is largely associated with vesicles in the organelle band, revealing a role for vesicles in plant meiosis and that cytoplasmic events maintain spindle position during the chromosome division.

PMID:
25757555
DOI:
10.1038/ncomms7492
[Indexed for MEDLINE]

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