Format

Send to

Choose Destination
Biomed Res Int. 2015;2015:635748. doi: 10.1155/2015/635748. Epub 2015 Feb 1.

MicroRNA and mRNA features of malignant pleural mesothelioma and benign asbestos-related pleural effusion.

Author information

1
Department of Chest Diseases, Medical Faculty, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey.
2
Division of Thoracic Surgery, Department of Surgery, Mayo Clinic, Rochester, MN 55905, USA.
3
Department of Molecular Medicine, Mayo Clinic, Rochester, MN 55905, USA.
4
Division of Oncology, National Jewish Health, Denver, CO 80206, USA.
5
Department of Public Health, Medical Faculty, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey.
6
Department of Pathology, Medical Faculty, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey.
7
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.
8
Division of Pulmonary & Critical Care Medicine, Mayo Clinic, Rochester, MN 55905, USA.

Abstract

INTRODUCTION:

We investigated the expression of microRNAs and mRNAs in pleural tissues from patients with either malignant pleural mesothelioma or benign asbestos-related pleural effusion.

METHODS:

Fresh frozen tissues from a total of 18 malignant pleural mesothelioma and 6 benign asbestos-related pleural effusion patients were studied. Expression profiling of mRNA and microRNA was performed using standard protocols.

RESULTS:

We discovered significant upregulation of multiple microRNAs in malignant pleural mesothelioma compared to benign asbestos-related pleural effusion. Hsa-miR-484, hsa-miR-320, hsa-let-7a, and hsa-miR-125a-5p were able to discriminate malignant from benign disease. Dynamically regulated mRNAs were also identified. MET was the most highly overexpressed gene in malignant pleural mesothelioma compared to benign asbestos-related pleural effusion. Integrated analyses examining microRNA-mRNA interactions suggested multiple altered targets within the Notch signaling pathway.

CONCLUSIONS:

Specific microRNAs and mRNAs may have diagnostic utility in differentiating patients with malignant pleural mesothelioma from benign asbestos-related pleural effusion. These studies may be particularly helpful in patients who reside in a region with a high incidence of mesothelioma.

PMID:
25756049
PMCID:
PMC4331157
DOI:
10.1155/2015/635748
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Hindawi Limited Icon for PubMed Central
Loading ...
Support Center