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Angew Chem Int Ed Engl. 2015 Apr 27;54(18):5369-73. doi: 10.1002/anie.201409597. Epub 2015 Mar 5.

Selective aptamer-based control of intraneuronal signaling.

Author information

1
Life and Medical Sciences Institute, University of Bonn, Gerhard-Domagk-Str. 1, Bonn (Germany).

Abstract

Cellular behavior is orchestrated by the complex interactions of a myriad of intracellular signal transduction pathways. To understand and investigate the role of individual components in such signaling networks, the availability of specific inhibitors is of paramount importance. We report the generation and validation of a novel variant of an RNA aptamer that selectively inhibits the mitogen-activated kinase pathway in neurons. We demonstrate that the aptamer retains function under intracellular conditions and that application of the aptamer through the patch-clamp pipette efficiently inhibits mitogen-activated kinase-dependent synaptic plasticity. This approach introduces synthetic aptamers as generic tools, readily applicable to inhibit different components of intraneuronal signaling networks with utmost specificity.

KEYWORDS:

MAP kinase; SELEX; aptamers; neurosciences

PMID:
25754968
PMCID:
PMC5324602
DOI:
10.1002/anie.201409597
[Indexed for MEDLINE]
Free PMC Article

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