Format

Send to

Choose Destination
Eur J Immunol. 2015 Jun;45(6):1706-15. doi: 10.1002/eji.201445421. Epub 2015 Apr 7.

ICOS is required for the generation of both central and effector CD4(+) memory T-cell populations following acute bacterial infection.

Author information

1
Institute for Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, UK.
2
Respiratory, Inflammation and Autoimmunity, Research Department, MedImmune LLC, Gaithersburg, MD, USA.

Abstract

Interactions between ICOS and ICOS ligand (ICOSL) are essential for the development of T follicular helper (Tfh) cells and thus the formation and maintenance of GC reactions. Given the conflicting reports on the requirement of other CD4(+) T-cell populations for ICOS signals, we have employed a range of in vivo approaches to dissect requirements for ICOS signals in mice during an endogenous CD4(+) T-cell response and contrasted this with CD28 signals. Genetic absence of ICOSL only modestly reduced the total number of antigen-specific CD4(+) T cells at the peak of the primary response, but resulted in a severely diminished number of both T central memory and T effector memory cells. Treatment with blocking anti-ICOS mAb during the primary response recapitulated these effects and caused a more substantial reduction than blocking CD28 signals with CTLA4Ig. During the memory phase of the response further signals through ICOS or CD28 were not required for survival. However, upon secondary challenge only Tfh cell expansion remained heavily ICOS-dependent, while CD28 signals were required for optimal expansion of all subsets. These data demonstrate the importance of ICOS signals specifically for memory CD4(+) T-cell formation, while highlighting the potential of therapeutically targeting this pathway.

KEYWORDS:

CD28; CD4+ Memory; ICOS; T-cell responses; Tfh

PMID:
25754933
PMCID:
PMC4736665
DOI:
10.1002/eji.201445421
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center