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Sci Rep. 2015 Mar 10;5:8932. doi: 10.1038/srep08932.

Lack of stress responses to long-term effects of corticosterone in Caps2 knockout mice.

Author information

1
1] Laboratory for Molecular Neurogenesis, RIKEN Brain Science Institute, Wako, Saitama, Japan [2] CREST, Kawaguchi, Saitama, Japan.
2
1] Laboratory for Molecular Neurogenesis, RIKEN Brain Science Institute, Wako, Saitama, Japan [2] CREST, Kawaguchi, Saitama, Japan [3] Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba, Japan.
3
1] Laboratory for Molecular Neurogenesis, RIKEN Brain Science Institute, Wako, Saitama, Japan [2] CREST, Kawaguchi, Saitama, Japan [3] Advanced Scientific Research Leaders Development Unit, Gunma University, Maebashi, Gunma, Japan.
4
1] CREST, Kawaguchi, Saitama, Japan [2] Health Research Institute, National Institute of Advanced Science and Technology, Ikeda, Osaka, Japan.
5
RIKEN BioResource Center, Tsukuba, Ibaraki, Japan.

Abstract

Chronic stress is associated with anxiety and depressive disorders, and can cause weight gain. Ca(2+)-dependent activator protein for secretion 2 (CAPS2) is involved in insulin release. Caps2 knockout (KO) mice exhibit decreased body weight, reduced glucose-induced insulin release, and abnormal psychiatric behaviors. We chronically administered the stress hormone corticosterone (CORT), which induces anxiety/depressive-like behavior and normally increases plasma insulin levels, via the drinking water for 10 weeks, and we examined the stress response in KO mice. Chronic CORT exposure inhibited stress-induced serum CORT elevation in wild-type (WT) mice, but not in KO mice. Poor weight gain in CORT-treated animals was observed until week 6 in WT mice, but persisted for the entire duration of the experiment in KO mice, although there is no difference in drug*genotype interaction. Among KO mice, food consumption was unchanged, while water consumption was higher, over the duration of the experiment in CORT-treated animals, compared with untreated animals. Moreover, serum insulin and leptin levels were increased in CORT-treated WT mice, but not in KO mice. Lastly, both WT and KO mice displayed anxiety/depressive-like behavior after CORT administration. These results suggest that Caps2 KO mice have altered endocrine responses to CORT administration, while maintaining CORT-induced anxiety/depressive-like behavior.

PMID:
25754523
PMCID:
PMC4354153
DOI:
10.1038/srep08932
[Indexed for MEDLINE]
Free PMC Article

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