Format

Send to

Choose Destination
Am J Reprod Immunol. 2015 Jun;73(6):582-8. doi: 10.1111/aji.12367. Epub 2015 Mar 4.

A New Enzyme-linked Sorbent Assay (ELSA) to Quantify Syncytiotrophoblast Extracellular Vesicles in Biological Fluids.

Author information

1
Placenta-Labor, Department of Obstetrics, University Hospital Jena, Jena, Germany.
2
Department of Obstetrics and Gynaecology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
3
Nuffield Department of Obstetrics and Gynaecology, Women's Center, University of Oxford, John Radcliffe Hospital, Oxford, UK.
4
Immunoendocrinology, Division of Medical Biology, Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.

Abstract

PROBLEM:

The pregnancy-associated disease preeclampsia is related to the release of syncytiotrophoblast extracellular vesicles (STBEV) by the placenta. To improve functional research on STBEV, reliable and specific methods are needed to quantify them. However, only a few quantification methods are available and accepted, though imperfect. For this purpose, we aimed to provide an enzyme-linked sorbent assay (ELSA) to quantify STBEV in fluid samples based on their microvesicle characteristics and placental origin.

METHOD OF STUDY:

Ex vivo placenta perfusion provided standards and samples for the STBEV quantification. STBEV were captured by binding of extracellular phosphatidylserine to immobilized annexin V. The membranous human placental alkaline phosphatase on the STBEV surface catalyzed a colorimetric detection reaction.

RESULTS AND CONCLUSION:

The described ELSA is a rapid and simple method to quantify STBEV in diverse liquid samples, such as blood or perfusion suspension. The reliability of the ELSA was proven by comparison with nanoparticle tracking analysis.

KEYWORDS:

Exosomes; microvesicles; placenta; placenta perfusion; preeclampsia; pregnancy

PMID:
25753333
DOI:
10.1111/aji.12367
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center