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Res Microbiol. 2015 Apr;166(3):205-14. doi: 10.1016/j.resmic.2015.02.010. Epub 2015 Mar 6.

Constitutive arsenite oxidase expression detected in arsenic-hypertolerant Pseudomonas xanthomarina S11.

Author information

1
UMR7156 Université de Strasbourg/CNRS Génétique Moléculaire, Génomique, Microbiologie, Département Micro-organismes, Génomes, Environnement, 28 rue Goethe, 67083 Strasbourg Cedex, France. Electronic address: sandrine.koechler@unistra.fr.
2
UMR7156 Université de Strasbourg/CNRS Génétique Moléculaire, Génomique, Microbiologie, Département Micro-organismes, Génomes, Environnement, 28 rue Goethe, 67083 Strasbourg Cedex, France. Electronic address: ploetze@unistra.fr.
3
Université de Lyon, Université Lyon 1, CNRS, UMR5558, Laboratoire de Biométrie et Biologie Evolutive, 43 boulevard du 11 Novembre 1918, F-69622 Villeurbanne, France. Electronic address: celine.brochier-armanet@univ-lyon1.fr.
4
UMR7156 Université de Strasbourg/CNRS Génétique Moléculaire, Génomique, Microbiologie, Département Micro-organismes, Génomes, Environnement, 28 rue Goethe, 67083 Strasbourg Cedex, France. Electronic address: florencegoulhen@yahoo.com.
5
UMR7156 Université de Strasbourg/CNRS Génétique Moléculaire, Génomique, Microbiologie, Département Micro-organismes, Génomes, Environnement, 28 rue Goethe, 67083 Strasbourg Cedex, France. Electronic address: salmeron_audrey@yahoo.fr.
6
Plateforme Biopuces et séquençage, IGBMC, 1 rue Laurent Fries Parc d'Innovation, 67400 Illkirch, France. Electronic address: jost@igbmc.fr.
7
UMR7156 Université de Strasbourg/CNRS Génétique Moléculaire, Génomique, Microbiologie, Département Micro-organismes, Génomes, Environnement, 28 rue Goethe, 67083 Strasbourg Cedex, France. Electronic address: didier.lievremont@unistra.fr.
8
Plateforme Biopuces et séquençage, IGBMC, 1 rue Laurent Fries Parc d'Innovation, 67400 Illkirch, France. Electronic address: mkoch@igbmc.fr.
9
UMR7156 Université de Strasbourg/CNRS Génétique Moléculaire, Génomique, Microbiologie, Département Micro-organismes, Génomes, Environnement, 28 rue Goethe, 67083 Strasbourg Cedex, France. Electronic address: f.plewniak@unistra.fr.
10
UMR7156 Université de Strasbourg/CNRS Génétique Moléculaire, Génomique, Microbiologie, Département Micro-organismes, Génomes, Environnement, 28 rue Goethe, 67083 Strasbourg Cedex, France. Electronic address: philippe.bertin@unistra.fr.
11
UMR7156 Université de Strasbourg/CNRS Génétique Moléculaire, Génomique, Microbiologie, Département Micro-organismes, Génomes, Environnement, 28 rue Goethe, 67083 Strasbourg Cedex, France. Electronic address: lett@unistra.fr.

Abstract

Pseudomonas xanthomarina S11 is an arsenite-oxidizing bacterium isolated from an arsenic-contaminated former gold mine in Salsigne, France. This bacterium showed high resistance to arsenite and was able to oxidize arsenite to arsenate at concentrations up to 42.72 mM As[III]. The genome of this strain was sequenced and revealed the presence of three ars clusters. One of them is located on a plasmid and is organized as an "arsenic island" harbouring an aio operon and genes involved in phosphorous metabolism, in addition to the ars genes. Neither the aioXRS genes nor a specific sigma-54-dependent promoter located upstream of aioBA genes, both involved in regulation of arsenite oxidase expression in other arsenite-oxidizing bacteria, could be identified in the genome. This observation is in accordance with the fact that no difference was observed in expression of arsenite oxidase in P. xanthomarina S11, whether or not the strain was grown in the presence of As[III].

KEYWORDS:

Arsenic tolerance; Genome; Metal; Resistance

PMID:
25753102
DOI:
10.1016/j.resmic.2015.02.010
[Indexed for MEDLINE]

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