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Trends Microbiol. 2015 Apr;23(4):192-203. doi: 10.1016/j.tim.2015.01.013. Epub 2015 Mar 5.

Towards an HIV-1 cure: measuring the latent reservoir.

Author information

  • 1Department of Medicine, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, MD 21205, USA.
  • 2Department of Medicine, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, MD 21205, USA; Howard Hughes Medical Institute, 733 North Broadway, Baltimore, MD 21205, USA. Electronic address: rsiliciano@jhmi.edu.

Abstract

The latent reservoir (LR) of HIV-1 in resting memory CD4(+) T cells serves as a major barrier to curing HIV-1 infection. While many PCR- and culture-based assays have been used to measure the size of the LR, correlation between results of different assays is poor and recent studies indicate that no available assay provides an accurate measurement of reservoir size. The discrepancies between assays are a hurdle to clinical trials that aim to measure the efficacy of HIV-1 eradication strategies. Here we describe the advantages and disadvantages of various approaches to measuring the LR.

KEYWORDS:

HIV-1; assays; cure; latency

PMID:
25747663
PMCID:
PMC4386620
DOI:
10.1016/j.tim.2015.01.013
[PubMed - indexed for MEDLINE]
Free PMC Article
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